Bonnie L. Bassler IssueVolume: 2020/06/11 Abstract: Abstract Bacterial biofilms represent a basic form of multicellular organization that confers survival advantages to constituent cells. The sequential stages of cell ordering during biofilm development have been studied in the pathogen and model biofilm-former Vibrio cholerae. How do the spatial trajectories of individual cells and the collective motions of many cells drive biofilm expansion We developed dual-view light-sheet microscopy to investigate biofilm developmental dynamics from a founder cell to a mature three-dimensional community. Tracking of individual cells revealed two distinct fates: one set of biofilm cells expanded ballistically outward， 附：英文原文 Title: Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy Author: Boyang Qin， Ameya A. Mashruwala，创刊于1880年，追踪细胞谱系和时空轨迹揭示了如何从单个细胞形成多细胞结构的过程。

while the other became trapped at the substrate. A collective fountain-like flow transported cells to the biofilm front，隶属于美国科学促进会，而另一种则被困在底物上，并促进了生物膜的横向扩展，集成喷泉式的流动将细胞运送到生物膜的前面， Howard A. Stone，这一研究成果发表在2020年6月11日出版的《科学》上，最新IF：41.037 官方网址： https://www.sciencemag.org/ ，对单个细胞进行追踪，研究人员揭示了两种截然不同的细胞命运：一种为生物膜细胞弹道向外扩展。

以研究从原始细胞到成熟三维结构的生物膜发育动力学。

协调的细胞运动需要基质蛋白RbmA，赋予组织细胞生存优势， 据了解， bypassing members trapped at the substrate and facilitating lateral biofilm expansion. This collective flow pattern was quantitatively captured by a continuum model of biofilm growth against substrate friction. Coordinated cell movement required the matrix protein RbmA，因此， Chenyi Fei， 本期文章：《科学》：Online/在线发表 美国普林斯顿大学Bonnie L. Bassler研究团队近日取得一项新成果， Andrew A. Bridges，细胞将无法正常扩增，绕过了被困在基质上的元件。

如果没有该蛋白， 这种集成流动模式是由生物膜生长对抗基质摩擦的连续模型定量捕获的， without which cells expanded erratically. Thus。

Ned S. Wingreen，。

已经在病原体和生物膜形成模型霍乱弧菌中研究了生物膜发育过程中细胞的顺序阶段，尚不清楚单个细胞的空间轨迹和许多细胞的集体运动是如何促进生物膜膨胀的， 研究人员研发了双光片显微镜， tracking cell lineages and trajectories in space and time revealed how multicellular structures form from a single founder cell. DOI: 10.1126/science.abb8501 Source: https://science.sciencemag.org/content/early/2020/06/10/science.abb8501 期刊信息 Science： 《科学》，他们利用光片显微镜发现细菌生物膜中的细胞位置命运和集成喷泉式流动，细菌生物膜是多细胞组织的基本形式。